Ida Höijer Cas9 off target sequencing and analysis

In this SMRT Leiden 2020 Online Virtual Event presentation, Ida Hoijer of Uppsala University shares her research on developing a long-read sequencing-based method for detection of CRISPR-Cas9 off-target effects, along with a customized analysis pipeline. By applying an off-target sequencing (OTS) method on a human embryonic cell line, they detected 55 on- and off-target sites for three different gRNAs, including allele-specific off-targets. As the OTS approach finds off-targets that are difficult to predict using in silico or short-read based methods, it may become an important tool for genome editing.
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CRISPR-Cas9,Gene editing,SMRT Sequencing,PacBio,Long read sequencing,Third generation sequencing,Single Molecule Real-Time,Pacific Biosciences,PacBio Systems,Next gen sequencing,Next generation sequencing,Science,Genomics,Genetics,Biology,